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foki assisted signal amplification assays  (New England Biolabs)


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    Structured Review

    New England Biolabs foki assisted signal amplification assays
    Foki Assisted Signal Amplification Assays, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/foki+assisted+signal+amplification+assays/FokI/10__1039_slash_d3sd00316g-42-7-23
    Average 95 stars, based on 479 article reviews
    foki assisted signal amplification assays - by Bioz Stars, 2026-11
    95/100 stars

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    Hybridization:

    Article Title: A FokI-driven signal amplification platform for the simultaneous detection of multiple viral RNA pathogens
    Article Snippet: Conventional hybridisation assays were performed using 2 μl of NEB 10× Cutsmart buffer (NEB #B7204), 100 nM of fluorescent dumbbell-like oligonucleotides targeting different viral targets, and decreasing concentrations of either target DNA, target RNA (5 nM, 1 nM, 500 pM, 100 pM, 50pM, 0 M) or control DNA/RNA (5 nM) for each respective reaction. .. In addition to the conventional hybridisation protocol, FokI-assisted signal amplification assays were performed by supplementing the reaction with 5 units of FokI RE (NEB, #R0109S). .. Fluorescence intensity measurements for these experiments were recorded using a StepOnePlusTM real-time PCR system (Applied Biosystems).

    Amplification:

    Article Title: A FokI-driven signal amplification platform for the simultaneous detection of multiple viral RNA pathogens
    Article Snippet: Conventional hybridisation assays were performed using 2 μl of NEB 10× Cutsmart buffer (NEB #B7204), 100 nM of fluorescent dumbbell-like oligonucleotides targeting different viral targets, and decreasing concentrations of either target DNA, target RNA (5 nM, 1 nM, 500 pM, 100 pM, 50pM, 0 M) or control DNA/RNA (5 nM) for each respective reaction. .. In addition to the conventional hybridisation protocol, FokI-assisted signal amplification assays were performed by supplementing the reaction with 5 units of FokI RE (NEB, #R0109S). .. Fluorescence intensity measurements for these experiments were recorded using a StepOnePlusTM real-time PCR system (Applied Biosystems).



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    95
    New England Biolabs foki assisted signal amplification assays
    Foki Assisted Signal Amplification Assays, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/foki+assisted+signal+amplification+assays/FokI/10__1039_slash_d3sd00316g-42-7-23
    Average 95 stars, based on 1 article reviews
    foki assisted signal amplification assays - by Bioz Stars, 2026-11
    95/100 stars
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